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Synaptoporin Polyclonal antibody

Synaptoporin Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14143-1-AP

Synonyms

SPO, synaptoporin, SYNPR

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Synaptoporin Polyclonal antibody (14143-1-AP)

    WB analysis using 14143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    WB analysis of human brain using 14143-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    WB analysis of mouse cerebellum using 14143-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14143-1-AP (Synaptoporin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IP experiment of mouse brain using 14143-1-AP

    IP result of anti-Synaptoporin (IP:14143-1-AP, 4ug; Detection:14143-1-AP 1:500) with mouse brain tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of mouse brain using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of mouse brain using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14143-1-AP (Synaptoporin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human colon using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human colon using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human gliomas using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human gliomas using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human brain using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Synaptoporin Polyclonal antibody (14143-1-AP)

    IHC staining of human brain using 14143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14143-1-AP (Synaptoporin antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

14143-1-AP targets Synaptoporin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptoporin fusion protein Ag5307
Full Name synaptoporin
Calculated Molecular Weight 265 aa, 29 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC022518
Gene Symbol SYNPR
Gene ID (NCBI) 132204
RRIDAB_2878022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Synaptoporin antibody 14143-1-APDownload protocol
IHC protocol for Synaptoporin antibody 14143-1-APDownload protocol
IP protocol for Synaptoporin antibody 14143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomedicines

Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome

Authors - Luisa Speranza
View Article
mouseIF

CNS Neurosci Ther

Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation

Authors - Limin Ma
View Article