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TBL2 Monoclonal antibody

TBL2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat no : 66307-1-Ig

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of MCF-7 using 66307-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of Jurkat using 66307-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBL2 Monoclonal antibody (66307-1-Ig)

    WB analysis of COLO 320 using 66307-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human liver using 66307-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human colon using 66307-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human colon using 66307-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TBL2 Monoclonal antibody (66307-1-Ig)

    IHC staining of human liver using 66307-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inMCF-7 cells, COLO 320 cells, Jurkat cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66307-1-Ig targets TBL2 in WB, IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen TBL2 fusion protein Ag24225
Full Name transducin (beta)-like 2
Calculated Molecular Weight 446 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC012938
Gene Symbol TBL2
Gene ID (NCBI) 26608
RRIDAB_2881689
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Protocols

Product Specific Protocols
WB protocol for TBL2 antibody 66307-1-IgDownload protocol
IHC protocol for TBL2 antibody 66307-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols