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TINP1 Polyclonal antibody

TINP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16230-1-AP

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TINP1 Polyclonal antibody (16230-1-AP)

    WB analysis using 16230-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16230-1-AP (TINP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using TINP1 Polyclonal antibody (16230-1-AP)

    WB analysis of Caco-2 using 16230-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 16230-1-AP (TINP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Polyclonal antibody (16230-1-AP)

    IHC staining of human breast cancer using 16230-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16230-1-AP (TINP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Polyclonal antibody (16230-1-AP)

    IHC staining of human breast cancer using 16230-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16230-1-AP (TINP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TINP1 Polyclonal antibody (16230-1-AP)

    IHC staining of human kidney using 16230-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16230-1-AP (TINP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16230-1-AP targets TINP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TINP1 fusion protein Ag8822
Full Name TGF beta-inducible nuclear protein 1
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC005288
Gene Symbol TINP1
Gene ID (NCBI) 10412
RRIDAB_2878232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

Protocols

Product Specific Protocols
WB protocol for TINP1 antibody 16230-1-APDownload protocol
IHC protocol for TINP1 antibody 16230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols