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TINP1 Monoclonal antibody

TINP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B11

Cat no : 66374-1-Ig

Synonyms

CDK105, Hairy cell leukemia protein 1, HCL G1, HUSSY29, NSA2, TINP1, YR 29

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of L02 using 66374-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of HEK-293 using 66374-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    WB analysis of HepG2 using 66374-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66374-1-Ig (TINP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Monoclonal antibody (66374-1-Ig)

    IHC staining of human breast cancer using 66374-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TINP1 Monoclonal antibody (66374-1-Ig)

    IHC staining of human breast cancer using 66374-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66374-1-Ig (TINP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    IF Staining of HEK-293 using 66374-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TINP1 Monoclonal antibody (66374-1-Ig)

    IF Staining of HEK-293 using 66374-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TINP1 antibody (66374-1-Ig, Clone: 1E3B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66374-1-Ig targets TINP1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TINP1 fusion protein Ag8822
Full Name TGF beta-inducible nuclear protein 1
Calculated Molecular Weight 260 aa, 30 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC005288
Gene Symbol TINP1
Gene ID (NCBI) 10412
RRIDAB_2881752
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression.

Protocols

Product Specific Protocols
WB protocol for TINP1 antibody 66374-1-IgDownload protocol
IHC protocol for TINP1 antibody 66374-1-IgDownload protocol
IF protocol for TINP1 antibody 66374-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols