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TMEM168 Polyclonal antibody

TMEM168 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22527-1-AP

Synonyms

TMEM168, transmembrane protein 168

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMEM168 Polyclonal antibody (22527-1-AP)

    WB analysis using 22527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22527-1-AP (TMEM168 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TMEM168 Polyclonal antibody (22527-1-AP)

    WB analysis of PC-3 using 22527-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22527-1-AP (TMEM168 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMEM168 Polyclonal antibody (22527-1-AP)

    IHC staining of human testis using 22527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22527-1-AP (TMEM168 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using TMEM168 Polyclonal antibody (22527-1-AP)

    IHC staining of human testis using 22527-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22527-1-AP (TMEM168 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse brain tissue, PC-3 cells, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22527-1-AP targets TMEM168 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM168 fusion protein Ag18337
Full Name transmembrane protein 168
Calculated Molecular Weight 697 aa, 80 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC030804
Gene Symbol TMEM168
Gene ID (NCBI) 64418
RRIDAB_2879118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM168 antibody 22527-1-APDownload protocol
IHC protocol for TMEM168 antibody 22527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

FASEB J

Identification of transmembrane protein 168 mutation in familial Brugada syndrome.

Authors - Akio Shimizu
View Article
humanIP, WB

J Biochem

Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics.

Authors - Le Kim Chi Nguyen
View Article

Oncol Res

Inhibition of proliferation by knockdown of transmembrane (TMEM) 168 in glioblastoma cells via suppression of Wnt/beta-catenin pathway.

Authors - Jie Xu
View Article