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TMEM182 Polyclonal antibody

TMEM182 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25366-1-AP

Synonyms

TMEM182, transmembrane protein 182

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TMEM182 Polyclonal antibody (25366-1-AP)

    WB analysis of mouse skeletal muscle using 25366-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25366-1-AP (TMEM182 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM182 Polyclonal antibody (25366-1-AP)

    IHC staining of human liver cancer using 25366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TMEM182 Polyclonal antibody (25366-1-AP)

    IHC staining of human liver cancer using 25366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25366-1-AP is TMEM182 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM182 fusion protein Ag21906
Full Name transmembrane protein 182
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 15 kDa 26 kDa
GenBank Accession NumberBC130308
Gene Symbol TMEM182
Gene ID (NCBI) 130827
RRIDAB_2880046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM182 antibody 25366-1-APDownload protocol
IHC protocol for TMEM182 antibody 25366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols