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TMEM208 Polyclonal antibody

TMEM208 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23882-1-AP

Synonyms

HSPC171, TMEM208, transmembrane protein 208

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using TMEM208 Polyclonal antibody (23882-1-AP)

    WB analysis of mouse brain using 23882-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23882-1-AP (TMEM208 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TMEM208 Polyclonal antibody (23882-1-AP)

    IHC staining of human stomach using 23882-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 23882-1-AP (TMEM208 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using TMEM208 Polyclonal antibody (23882-1-AP)

    IHC staining of human stomach using 23882-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 23882-1-AP (TMEM208 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23882-1-AP is TMEM208 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM208 fusion protein Ag20845
Full Name transmembrane protein 208
Calculated Molecular Weight 173 aa, 20 kDa
Observed Molecular Weight 19-29 kDa
GenBank Accession NumberBC003080
Gene Symbol TMEM208
Gene ID (NCBI) 29100
RRIDAB_2879349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 208 (TMEM208) can regulate both autophagy and ER stress. When overexpressed, TMEM208 impaired autophagy as characterized by the decrease of the accumulation of LC3-II, decreased degradation of autophagic substrates, and reduced expression of critical effectors and vital molecules of the ER stress and autophagy processes. (PMID: 23691174)

Protocols

Product Specific Protocols
WB protocol for TMEM208 antibody 23882-1-APDownload protocol
IHC protocol for TMEM208 antibody 23882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Transmembrane insertases and N-glycosylation critically determine synthesis, trafficking and activity of the nonselective cation channel TRPC6.

Authors - Brianna E Talbot
View Article
humanWB

Nat Cancer

Targeting LIPA independent of its lipase activity is a therapeutic strategy in solid tumors via induction of endoplasmic reticulum stress

Authors - Xihui Liu
View Article
humanWB

Cell Rep

Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins

Authors - Jicheng Yang
  • KO Validated
View Article