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TMEM24 Polyclonal antibody

TMEM24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25507-1-AP

Synonyms

C2CD2 like, C2CD2L, DLNB23, KIAA0285, TMEM24, Transmembrane protein 24

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TMEM24 Polyclonal antibody (25507-1-AP)

    WB analysis of HeLa using 25507-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25507-1-AP (TMEM24 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TMEM24 Polyclonal antibody (25507-1-AP)

    IHC staining of human pancreas cancer using 25507-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TMEM24 Polyclonal antibody (25507-1-AP)

    IHC staining of human pancreas cancer using 25507-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 25507-1-AP is TMEM24 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM24 fusion protein Ag21913
Full Name C2CD2-like
Calculated Molecular Weight 706 aa, 76 kDa
Observed Molecular Weight 80-100 kDa
GenBank Accession NumberBC022219
Gene Symbol C2CD2L
Gene ID (NCBI) 9854
RRIDAB_2880108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM24, also named as KIAA0285 and C2CD2L, is identified as a pancreatic islet-enriched protein that plays a key role in regulating glucose-sensitive INS release from the reserve pool of granules. This antibody detects TMEM24 protein with MW 70-100 kDa.

Protocols

Product Specific Protocols
WB protocol for TMEM24 antibody 25507-1-APDownload protocol
IHC protocol for TMEM24 antibody 25507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article