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TOM1 Polyclonal antibody

TOM1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17506-1-AP

Synonyms

Target of Myb protein 1, target of myb1 (chicken), TOM1

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TOM1 Polyclonal antibody (17506-1-AP)

    WB analysis of human liver using 17506-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17506-1-AP (TOM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using TOM1 Polyclonal antibody (17506-1-AP)

    WB analysis of human skeletal muscle using 17506-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17506-1-AP (TOM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TOM1 Polyclonal antibody (17506-1-AP)

    WB analysis of K-562 using 17506-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17506-1-AP (TOM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TOM1 Polyclonal antibody (17506-1-AP)

    WB analysis of HeLa using 17506-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17506-1-AP (TOM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman liver tissue, HeLa cells, human skeletal muscle tissue, K-562 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17506-1-AP targets TOM1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM1 fusion protein Ag11706
Full Name target of myb1 (chicken)
Calculated Molecular Weight 493 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC046151
Gene Symbol TOM1
Gene ID (NCBI) 10043
RRIDAB_2207412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOM1 is a member of VHS (VPS-27, Hrs, and STAM)-domain-containing protein family that functions as adaptor proteins in the intracellular trafficking and sorting of plasma membrane proteins. TOM1 colocalized with key proteins of the protein-degradation systems. Recently TOM1 has been reported to be localized in the hallmarks of AD pathology (27206884).

Protocols

Product Specific Protocols
WB protocol for TOM1 antibody 17506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

J Neurol Sci

Immunolocalization of Tom1 in relation to protein degradation systems in Alzheimer's disease.

Authors - Kouki Makioka
View Article