- Featured Product
- KD/KO Validated
TOP2A Polyclonal antibody
TOP2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB,IP,IHC,IF,ELISA
Conjugate
Unconjugated
Cat no : 24641-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
24641-1-AP targets TOP2A in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOP2A fusion protein Ag19551 |
| Full Name | topoisomerase (DNA) II alpha 170kDa |
| Calculated Molecular Weight | 1531 aa, 174 kDa |
| Observed Molecular Weight | 174 kDa |
| GenBank Accession Number | BC140791 |
| Gene Symbol | TOP2A |
| Gene ID (NCBI) | 7153 |
| RRID | AB_2879651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOP2A antibody 24641-1-AP | Download protocol |
| IHC protocol for TOP2A antibody 24641-1-AP | Download protocol |
| IF protocol for TOP2A antibody 24641-1-AP | Download protocol |
| IP protocol for TOP2A antibody 24641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC. | ||
Mol Biol Cell SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex. | ||
Dev Biol Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes. | ||
Chem Biol Interact SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. | ||
Oncol Rep Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study. | ||
J Clin Neurosci High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas. |
