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TPP1 Polyclonal antibody

TPP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12479-1-AP

Synonyms

CLN2, GIG1, LPIC, TPP 1, TPP I, TPP1, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 1, tripeptidyl peptidase I, UNQ267/PRO304

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using TPP1 Polyclonal antibody (12479-1-AP)

    WB analysis of human colon using 12479-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12479-1-AP (TPP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of mouse liver using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12479-1-AP (TPP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of mouse liver using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12479-1-AP (TPP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPP1 Polyclonal antibody (12479-1-AP)

    IHC staining of human colon cancer using 12479-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12479-1-AP targets TPP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPP1 fusion protein Ag3173
Full Name tripeptidyl peptidase I
Calculated Molecular Weight 563 aa, 61 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC014863
Gene Symbol TPP1
Gene ID (NCBI) 1200
RRIDAB_2205902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIPEPTIDYL PEPTIDASE I, TPP1, is a lysosomal exopeptidase that sequentially removes tripeptides from the N termini of proteins. The proenzyme has an apparent molecular mass of 66 kD, and the mature enzyme has an apparent molecular mass of 46 to 48 kD. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). This protein distributes widely in all tissues.

Protocols

Product Specific Protocols
WB protocol for TPP1 antibody 12479-1-APDownload protocol
IHC protocol for TPP1 antibody 12479-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Reprod Dev

Protein profiling the differences between diabetic and normal mouse cumulus cells.

Authors - Guang-Jian Jiang
View Article