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  • Featured Product
  • KD/KO Validated

TWF1 Polyclonal antibody

TWF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11732-1-AP

Synonyms

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWF1 Polyclonal antibody (11732-1-AP)

    WB analysis using 11732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TWF1 Polyclonal antibody (11732-1-AP)

    WB analysis using 11732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TWF1 Polyclonal antibody (11732-1-AP)

    WB analysis of HeLa using 11732-1-AP

    WB result of TWF1 antibody (11732-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TWF1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TWF1 Polyclonal antibody (11732-1-AP)

    IHC staining of human lung cancer using 11732-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TWF1 Polyclonal antibody (11732-1-AP)

    IHC staining of human breast cancer using 11732-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TWF1 Polyclonal antibody (11732-1-AP)

    IF Staining of HeLa using 11732-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TWF1 antibody (11732-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inC6 cells, HeLa cells, C2C12 cells, mouse testis tissue, rat testis tissue, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11732-1-AP targets TWF1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWF1 fusion protein Ag2332
Full Name twinfilin, actin-binding protein, homolog 1 (Drosophila)
Calculated Molecular Weight 368 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC022344
Gene Symbol TWF1
Gene ID (NCBI) 5756
RRIDAB_2241259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TWF1 antibody 11732-1-APDownload protocol
IHC protocol for TWF1 antibody 11732-1-APDownload protocol
IF protocol for TWF1 antibody 11732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biochem Biophys Res Commun

Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.

Authors - Lu Li
  • KD Validated
View Article
humanWB

J Thorac Dis

Overexpression of TWF1 promotes lung adenocarcinoma progression and is associated with poor prognosis in cancer patients through the MMP1 signaling pathway

Authors - Kui Zhai
  • KD Validated
View Article
human,mouseWB,IHC,CoIP

FASEB J

TWF1 induces autophagy and accelerates malignant phenotype in lung adenocarcinoma via inhibiting the cAMP signaling pathway

Authors - Yu Wang
  • KD Validated
View Article