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TWSG1 Polyclonal antibody

TWSG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12327-1-AP

Synonyms

TSG, TWSG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWSG1 Polyclonal antibody (12327-1-AP)

    WB analysis using 12327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12327-1-AP (TWSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TWSG1 Polyclonal antibody (12327-1-AP)

    IHC staining of human kidney using 12327-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TWSG1 Polyclonal antibody (12327-1-AP)

    IHC staining of mouse brain using 12327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TWSG1 Polyclonal antibody (12327-1-AP)

    IHC staining of human kidney using 12327-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TWSG1 Polyclonal antibody (12327-1-AP)

    IHC staining of mouse brain using 12327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12327-1-AP targets TWSG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWSG1 fusion protein Ag2983
Full Name twisted gastrulation homolog 1 (Drosophila)
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC020490
Gene Symbol TWSG1
Gene ID (NCBI) 57045
RRIDAB_2877845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic proteins (BMPs) are potent morphogens that are involved in cell fate determination, proliferation, apoptosis and adult tissue homeostasis. Twisted gastrulation (TWSG1) is a secreted BMP binding protein that modulates BMP ligand availability in the extracellular space. Several in vitro studies point toward an important role of TWSG1 in the regulation of the immune system (PMID: 21572028).

Protocols

Product Specific Protocols
WB protocol for TWSG1 antibody 12327-1-APDownload protocol
IHC protocol for TWSG1 antibody 12327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols