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UBE2Q2 Polyclonal antibody

UBE2Q2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12581-1-AP

Synonyms

UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using UBE2Q2 Polyclonal antibody (12581-1-AP)

    WB analysis of A431 using 12581-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using UBE2Q2 Polyclonal antibody (12581-1-AP)

    WB analysis of HEK-293T using 12581-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using UBE2Q2 Polyclonal antibody (12581-1-AP)

    WB analysis of mouse ovary using 12581-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using UBE2Q2 Polyclonal antibody (12581-1-AP)

    WB analysis of A431 using 12581-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using UBE2Q2 Polyclonal antibody (12581-1-AP)

    WB analysis of mouse ovary using 12581-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using UBE2Q2 Polyclonal antibody (12581-1-AP)

    IP experiment of HEK-293T using 12581-1-AP

    IP result of anti-UBE2Q2 (IP:12581-1-AP, 4ug; Detection:12581-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using UBE2Q2 Polyclonal antibody (12581-1-AP)

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using UBE2Q2 Polyclonal antibody (12581-1-AP)

    IHC staining of human skin cancer using 12581-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, mouse ovary tissue, HEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12581-1-AP targets UBE2Q2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2Q2 fusion protein Ag3276
Full Name ubiquitin-conjugating enzyme E2Q family member 2
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC017708
Gene Symbol UBE2Q2
Gene ID (NCBI) 92912
RRIDAB_2256807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IHC protocol for UBE2Q2 antibody 12581-1-APDownload protocol
IP protocol for UBE2Q2 antibody 12581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
View Article