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UBR1 Polyclonal antibody

UBR1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26069-1-AP

Synonyms

JBS, N recognin 1, UBR1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using UBR1 Polyclonal antibody (26069-1-AP)

    IP experiment of mouse skeletal muscle using 26069-1-AP

    IP result of anti-UBR1 (IP:26069-1-AP, 4ug; Detection:26069-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR1 Polyclonal antibody (26069-1-AP)

    IHC staining of human pancreas using 26069-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using UBR1 Polyclonal antibody (26069-1-AP)

    IHC staining of human pancreas using 26069-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26069-1-AP (UBR1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26069-1-AP targets UBR1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBR1 fusion protein Ag22076
Full Name ubiquitin protein ligase E3 component n-recognin 1
Calculated Molecular Weight 1749 aa, 200 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC113505
Gene Symbol UBR1
Gene ID (NCBI) 197131
RRIDAB_2880360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for UBR1 antibody 26069-1-APDownload protocol
IP protocol for UBR1 antibody 26069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

J Cachexia Sarcopenia Muscle

Identification of Ubr1 as an amino acid sensor of steatosis in liver and muscle

Authors - Wanni Zhao
View Article
humanWB

Diabetes

N-end Rule-Mediated Proteasomal Degradation of ATGL Promotes Lipid Storage

Authors - Jiesi Xu
  • KD Validated
View Article