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  • Featured Product
  • KD/KO Validated

UFD1L Polyclonal antibody

UFD1L Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10615-1-AP

Synonyms

UB fusion protein 1, UFD1, UFD1L

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using UFD1L Polyclonal antibody (10615-1-AP)

    WB analysis of K-562 using 10615-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using UFD1L Polyclonal antibody (10615-1-AP)

    WB analysis of human testis using 10615-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using UFD1L Polyclonal antibody (10615-1-AP)

    WB analysis of mouse thymus using 10615-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UFD1L Polyclonal antibody (10615-1-AP)

    WB analysis of HepG2 using 10615-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using UFD1L Polyclonal antibody (10615-1-AP)

    WB analysis of Raji using 10615-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using UFD1L Polyclonal antibody (10615-1-AP)

    IP experiment of Raji using 10615-1-AP

    IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UFD1L Polyclonal antibody (10615-1-AP)

    IF Staining of HeLa using 10615-1-AP

    Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UFD1L Polyclonal antibody (10615-1-AP)

    IF Staining of HepG2 using 10615-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using UFD1L Polyclonal antibody (10615-1-AP)

    FC experiment of HEK-293 using 10615-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inK-562 cells, mouse thymus tissue, Raji cells, HepG2 cells, human testis tissue
Positive IP detected inRaji cells
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10615-1-AP targets UFD1L in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UFD1L fusion protein Ag0954
Full Name ubiquitin fusion degradation 1 like (yeast)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC005087
Gene Symbol UFD1L
Gene ID (NCBI) 7353
RRIDAB_2213944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UFD1L antibody 10615-1-APDownload protocol
IF protocol for UFD1L antibody 10615-1-APDownload protocol
IP protocol for UFD1L antibody 10615-1-APDownload protocol
FC protocol for UFD1L antibody 10615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Science

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Authors - Youngdae Gwon
View Article
humanWB

Nature

ESCRT-III controls nuclear envelope reformation.

Authors - Yolanda Olmos
  • KD Validated
View Article
humanWB

Dev Cell

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

Authors - Samuel S Wallis
  • KD Validated
View Article
humanWB

Elife

The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP

Authors - Maria Körner
View Article
mouseWB

Sci Signal

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing

Authors - Liangbin Lin
View Article
WB

Plant J

The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.

Authors - Peifeng Yu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Leslie (Verified Customer) (10-08-2019)

Excellent antibody for Western blots

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Skeletal Muscle
UFD1L Antibody Western Blot, validation (1:1000 dilution) in Skeletal Muscle (Cat no:10615-1-AP)