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USP8 Monoclonal antibody

USP8 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (1)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E12E3

Cat no : 67321-1-Ig

Synonyms

Deubiquitinating enzyme 8, FLJ34456, hUBPy, HumORF8, KIAA0055, Ubiquitin isopeptidase Y, ubiquitin specific peptidase 8, Ubiquitin thiolesterase 8, UBPY, USP8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP8 Monoclonal antibody (67321-1-Ig)

    WB analysis using 67321-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using USP8 Monoclonal antibody (67321-1-Ig)

    WB analysis of NIH/3T3 using 67321-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using USP8 Monoclonal antibody (67321-1-Ig)

    WB analysis of NIH/3T3 using 67321-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using USP8 Monoclonal antibody (67321-1-Ig)

    WB analysis using 67321-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using USP8 Monoclonal antibody (67321-1-Ig)

    WB analysis using 67321-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67321-1-Ig (USP8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP8 Monoclonal antibody (67321-1-Ig)

    IHC staining of human breast cancer using 67321-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using USP8 Monoclonal antibody (67321-1-Ig)

    IHC staining of human breast cancer using 67321-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67321-1-Ig (USP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using USP8 Monoclonal antibody (67321-1-Ig)

    IF Staining of HeLa using 67321-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP8 antibody (67321-1-Ig, Clone: 1E12E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of Hela cells using USP8 Monoclonal antibody (67321-1-Ig)

    FC experiment of Hela using 67321-1-Ig

    1X10^6 Hela cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using USP8 Monoclonal antibody (67321-1-Ig)

    FC experiment of HeLa using 67321-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human USP8 (67321-1-Ig, Clone:1E12E3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, LNcaP cells, Jurkat cells, K-562 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67321-1-Ig targets USP8 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP8 fusion protein Ag27104
Full Name ubiquitin specific peptidase 8
Calculated Molecular Weight 1118 aa, 128 kDa
Observed Molecular Weight 128 kDa
GenBank Accession NumberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
RRIDAB_2882581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for USP8 antibody 67321-1-IgDownload protocol
IHC protocol for USP8 antibody 67321-1-IgDownload protocol
IF protocol for USP8 antibody 67321-1-IgDownload protocol
FC protocol for USP8 antibody 67321-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization

Authors - Jianing Tang
View Article
humanWB

J Hum Genet

A heterozygous germline deletion within USP8 causes severe neurodevelopmental delay with multiorgan abnormalities

Authors - Masamune Sakamoto
View Article
humanWB

J Adv Res

The ubiquitin-specific protease 8 antagonizes melatonin-induced endocytic degradation of MT(1) receptor to promote lung adenocarcinoma growth

Authors - Qianhui Sun
  • KD Validated
View Article
humanWB,IF,IP,CoIP

Adv Sci (Weinh)

Targeting USP8 Inhibits O-GlcNAcylation of SLC7A11 to Promote Ferroptosis of Hepatocellular Carcinoma via Stabilization of OGT

Authors - Jianing Tang
View Article