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  • Featured Product
  • KD/KO Validated

VAT1 Polyclonal antibody

VAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22016-1-AP

Synonyms

VAT1, VATI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAT1 Polyclonal antibody (22016-1-AP)

    WB analysis using 22016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using VAT1 Polyclonal antibody (22016-1-AP)

    WB analysis of MCF-7 using 22016-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using VAT1 Polyclonal antibody (22016-1-AP)

    WB analysis of Jurkat using 22016-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using VAT1 Polyclonal antibody (22016-1-AP)

    WB analysis of C6 using 22016-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 22016-1-AP (VAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using VAT1 Polyclonal antibody (22016-1-AP)

    IHC staining of human gliomas using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using VAT1 Polyclonal antibody (22016-1-AP)

    IHC staining of human gliomas using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using VAT1 Polyclonal antibody (22016-1-AP)

    IHC staining of human brain using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using VAT1 Polyclonal antibody (22016-1-AP)

    IHC staining of human brain using 22016-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 22016-1-AP (VAT1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inC6 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22016-1-AP is VAT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAT1 fusion protein Ag16652
Full Name vesicle amine transport protein 1 homolog (T. californica)
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC015041
Gene Symbol VAT1
Gene ID (NCBI) 10493
RRIDAB_11182282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VAT1 antibody 22016-1-APDownload protocol
IHC protocol for VAT1 antibody 22016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Cancer Immunol Immunother

Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.

Authors - Pei Yang
  • KD Validated
View Article
humanWB

Oncol Rep

High expression of VAT1 is a prognostic biomarker and predicts malignancy in glioblastoma.

Authors - Xia Shan
View Article