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VBP1 Polyclonal antibody

VBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14345-1-AP

Synonyms

HIBBJ46, PFD3, PFDN3, Prefoldin subunit 3, VBP 1, VBP1, VHL binding protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VBP1 Polyclonal antibody (14345-1-AP)

    WB analysis using 14345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:8000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse liver tissue using VBP1 Polyclonal antibody (14345-1-AP)

    WB analysis of mouse liver using 14345-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using VBP1 Polyclonal antibody (14345-1-AP)

    WB analysis of K-562 using 14345-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VBP1 Polyclonal antibody (14345-1-AP)

    WB analysis of HepG2 using 14345-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using VBP1 Polyclonal antibody (14345-1-AP)

    IHC staining of human lymphoma using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using VBP1 Polyclonal antibody (14345-1-AP)

    IHC staining of human lymphoma using 14345-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VBP1 Polyclonal antibody (14345-1-AP)

    IF Staining of HepG2 using 14345-1-AP

    Immunofluorescent analysis of HepG2 cells, using VBP1 antibody 14345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inC6 cells, K-562 cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

14345-1-AP targets VBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VBP1 fusion protein Ag5685
Full Name von Hippel-Lindau binding protein 1
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC046094
Gene Symbol VBP1
Gene ID (NCBI) 7411
RRIDAB_2213910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VBP1 antibody 14345-1-APDownload protocol
IHC protocol for VBP1 antibody 14345-1-APDownload protocol
IF protocol for VBP1 antibody 14345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

PLoS Genet

The prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation.

Authors - Franck Chesnel
View Article