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Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E4D10

Cat no : 66175-1-Ig

Synonyms

DBP, DBP/GC, GC, Gc globulin, VDB, VDBG, VDBP, VDBP,GC, Vitamin D binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    WB analysis of human testis using 66175-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-937 cells using Vitamin D binding protein Monoclonal antibody (66175-1-Ig)

    FC experiment of U-937 using 66175-1-Ig

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman testis tissue
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66175-1-Ig targets Vitamin D binding protein in WB, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vitamin D binding protein fusion protein Ag9803
Full Name group-specific component (vitamin D binding protein)
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC057228
Gene Symbol GC
Gene ID (NCBI) 2638
RRIDAB_2881570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Protocols

Product Specific Protocols
WB protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
FC protocol for Vitamin D binding protein antibody 66175-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF,WB,CoIP,ChIP

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
View Article