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WASF3 Monoclonal antibody

WASF3 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B7D10

Cat no : 67620-1-Ig

Synonyms

Brush 1, KIAA0900, Protein WAVE 3, SCAR3, WAS protein family, member 3, WASF3, WASP family protein member 3, WAVE3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WASF3 Monoclonal antibody (67620-1-Ig)

    WB analysis using 67620-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67620-1-Ig (WASF3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IHC staining of human liver cancer using 67620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IHC staining of human liver cancer using 67620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IHC staining of human liver cancer using 67620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IHC staining of human liver cancer using 67620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67620-1-Ig (WASF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IF Staining of mouse brain using 67620-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using WASF3 Monoclonal antibody (67620-1-Ig)

    IF Staining of human liver cancer using 67620-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using WASF3 antibody (67620-1-Ig, Clone: 3B7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, human liver cancer tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67620-1-Ig targets WASF3 in IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WASF3 fusion protein Ag29991
Full Name WAS protein family, member 3
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 and 66-70 kDa
GenBank Accession NumberBC050283
Gene Symbol WASF3
Gene ID (NCBI) 10810
RRIDAB_2882822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASF3, also named WAVE3 or SCAR3, belongs to the SCAR/WAVE family. It plays a role in the regulation of cell morphology and cytoskeletal organization. WASF3 is associated with movement, invasion, and metastasis in different cancers such as breast cancer, gastric cancer, and prostate cancer. It has been shown that WASF3 levels are elevated in advanced-stage cancers compared to lower-grade tumors and normal tissue. The 67620-1-Ig antibody recognizes 50-55 kDa and 66-70 kDa proteins similar to the papers.(PMID: 21544801, 15826941, 31038356, 27432794, 31542393)

Protocols

Product Specific Protocols
WB protocol for WASF3 antibody 67620-1-IgDownload protocol
IHC protocol for WASF3 antibody 67620-1-IgDownload protocol
IF protocol for WASF3 antibody 67620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols