• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WASF4-Specific Polyclonal antibody

WASF4-Specific Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19755-1-AP

Synonyms

WAS protein family, member 4, WASF4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using WASF4-Specific Polyclonal antibody (19755-1-AP)

    WB analysis of mouse brain using 19755-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using WASF4-Specific Polyclonal antibody (19755-1-AP)

    WB analysis of mouse brain using 19755-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19755-1-AP (WASF4-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WASF4-Specific Polyclonal antibody (19755-1-AP)

    IF Staining of HepG2 using 19755-1-AP

    Immunofluorescent analysis of HepG2 cells, using WASF4 antibody 19755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19755-1-AP targets WASF4-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name WAS protein family, member 4
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberQ8IV90
Gene Symbol WASF4
Gene ID (NCBI) 644739
RRIDAB_10646483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WASF4, also named as SCAR2, belongs to the SCAR/WAVE family. WASF4 is a downstream effector molecule which involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. The antibody is specific to WASF4.

Protocols

Product Specific Protocols
WB protocol for WASF4-Specific antibody 19755-1-APDownload protocol
IF protocol for WASF4-Specific antibody 19755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols