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WDFY3 / ALFY Polyclonal antibody

WDFY3 / ALFY Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25134-1-AP

Synonyms

ALFY, Autophagy linked FYVE protein, KIAA0993, WDFY3, ZFYVE25

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WDFY3 / ALFY Polyclonal antibody (25134-1-AP)

    IHC staining of human breast cancer using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WDFY3 / ALFY Polyclonal antibody (25134-1-AP)

    IHC staining of human breast cancer using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using WDFY3 / ALFY Polyclonal antibody (25134-1-AP)

    IHC staining of human ovary tumor using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using WDFY3 / ALFY Polyclonal antibody (25134-1-AP)

    IHC staining of human ovary tumor using 25134-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25134-1-AP (WDFY3 / ALFY antibody at dilution of 1:100 (under 40x lens).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25134-1-AP is WDFY3 / ALFY Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDFY3 / ALFY fusion protein Ag18393
Full Name WD repeat and FYVE domain containing 3
Calculated Molecular Weight 3526 aa, 395 kDa
GenBank Accession NumberBC013377
Gene Symbol WDFY3
Gene ID (NCBI) 23001
RRIDAB_2879916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDFY3 also called ALFY, is a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy.This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.

Protocols

Product Specific Protocols
IHC protocol for WDFY3 / ALFY antibody 25134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols