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XYLB Polyclonal antibody

XYLB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26541-1-AP

Synonyms

XYLB, Xylulokinase, Xylulose kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using XYLB Polyclonal antibody (26541-1-AP)

    WB analysis of A431 using 26541-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 26541-1-AP (XYLB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using XYLB Polyclonal antibody (26541-1-AP)

    IHC staining of human skin cancer using 26541-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using XYLB Polyclonal antibody (26541-1-AP)

    IHC staining of human skin cancer using 26541-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 26541-1-AP (XYLB Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26541-1-AP is XYLB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XYLB fusion protein Ag19208
Full Name xylulokinase homolog (H. influenzae)
Calculated Molecular Weight 536 aa, 58 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC137076
Gene Symbol XYLB
Gene ID (NCBI) 9942
RRIDAB_2880546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human xylulokinase-like protein (XYLB), is a 58 kDa protein belongs to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. XYLB is a key regulator for controlling metabolic disease.XYLB also plays important roles in energy metabolism. XYLB has 2 isoforms produced by alternative splicing with the MW of 58 and 43 kDa.

Protocols

Product Specific Protocols
WB protocol for XYLB antibody 26541-1-APDownload protocol
IHC protocol for XYLB antibody 26541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols