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- KD/KO Validated
YOD1 Polyclonal antibody
YOD1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25370-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
The immunogen of 25370-1-AP is YOD1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YOD1 fusion protein Ag21881 |
| Full Name | YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 348 aa, 38 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC137167 |
| Gene Symbol | YOD1 |
| Gene ID (NCBI) | 55432 |
| RRID | AB_2880048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YOD1 antibody 25370-1-AP | Download protocol |
| IHC protocol for YOD1 antibody 25370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators | ||
Cell Signal Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis | ||
Biomed Res Int Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway.
| ||
Biomed Environ Sci Overexpression of YOD1 Promotes the Migration of Human Oral Keratinocytes by Enhancing TGF-β3 Signaling. | ||
Biochem Biophys Res Commun Deubiquitinating enzyme YOD1 deubiquitinates and destabilizes α-synuclein |
