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ZFYVE19 Polyclonal antibody

ZFYVE19 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23163-1-AP

Synonyms

MPFYVE, ZFYVE19

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZFYVE19 Polyclonal antibody (23163-1-AP)

    WB analysis of HeLa using 23163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23163-1-AP (ZFYVE19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ZFYVE19 Polyclonal antibody (23163-1-AP)

    WB analysis of Raji using 23163-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23163-1-AP (ZFYVE19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using ZFYVE19 Polyclonal antibody (23163-1-AP)

    IP experiment of Raji using 23163-1-AP

    IP result of anti-ZFYVE19 (IP:23163-1-AP, 4ug; Detection:23163-1-AP 1:2000) with Raji cells lysate 3880ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using ZFYVE19 Polyclonal antibody (23163-1-AP)

    IHC staining of human heart using 23163-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23163-1-AP (ZFYVE19 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ZFYVE19 Polyclonal antibody (23163-1-AP)

    IHC staining of human liver cancer using 23163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23163-1-AP (ZFYVE19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ZFYVE19 Polyclonal antibody (23163-1-AP)

    IHC staining of human liver cancer using 23163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23163-1-AP (ZFYVE19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using ZFYVE19 Polyclonal antibody (23163-1-AP)

    IHC staining of human heart using 23163-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23163-1-AP (ZFYVE19 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23163-1-AP is ZFYVE19 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE19 fusion protein Ag19593
Full Name zinc finger, FYVE domain containing 19
Calculated Molecular Weight 471 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015738
Gene Symbol ZFYVE19
Gene ID (NCBI) 84936
RRIDAB_2879219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZFYVE19 antibody 23163-1-APDownload protocol
IHC protocol for ZFYVE19 antibody 23163-1-APDownload protocol
IP protocol for ZFYVE19 antibody 23163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols