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ZFYVE28 Polyclonal antibody

ZFYVE28 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25360-1-AP

Synonyms

hLst2, KIAA1643, LST2, ZFYVE28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZFYVE28 Polyclonal antibody (25360-1-AP)

    WB analysis using 25360-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25360-1-AP (ZFYVE28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25360-1-AP targets ZFYVE28 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZFYVE28 fusion protein Ag19796
Full Name zinc finger, FYVE domain containing 28
Calculated Molecular Weight 887 aa, 96 kDa
Observed Molecular Weight 96 kDa
GenBank Accession NumberBC137309
Gene Symbol ZFYVE28
Gene ID (NCBI) 57732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.

Protocols

Product Specific Protocols
WB protocol for ZFYVE28 antibody 25360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols