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luciferase Monoclonal antibody

luciferase Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

　 and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C12

Cat no : 60158-1-Ig

Synonyms

dNGLUC, Luciferase

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using luciferase Monoclonal antibody (60158-1-Ig)

    WB analysis of Recombinant protein using 60158-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60158-1-Ig (dNGLUC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

60158-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with 　 samples.

Tested Reactivity 　
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen luciferase fusion protein Ag13000
Full Name luciferase
Calculated Molecular Weight 20 kDa
GenBank Accession NumberAY015993
Gene Symbol
Gene ID (NCBI)
RRIDAB_10665950
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The immunogen of this antibody the luciferase(AY015993) from the copepod marine organism Gaussia princeps. This antibody is a McAb to Luciferase.

Protocols

Product Specific Protocols
WB protocol for luciferase antibody 60158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Multiplex Assay for Live-Cell Monitoring of Cellular Fates of Amyloid-β Precursor Protein (APP).

Authors - Maria Merezhko
View Article
humanWB

Mol Ther

Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization

Authors - Yuanjun Zhu
View Article