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humanin Polyclonal antibody

humanin Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25886-1-AP

Synonyms

humanin, l rRNA, MTRNR2, RNR2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human colon using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human colon using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human heart using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human heart using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human small intestine using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using humanin Polyclonal antibody (25886-1-AP)

    IHC staining of human small intestine using 25886-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25886-1-AP (humanin Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using humanin Polyclonal antibody (25886-1-AP)

    FC experiment of SH-SY5Y using 25886-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug humanin antibody (25886-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive IHC detected inhuman colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

25886-1-AP targets humanin in FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name l-rRNA
GenBank Accession NumberNC_012920
Gene Symbol RNR2
Gene ID (NCBI) 4550
RRIDAB_2880282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Humanin, also named as MT-RNR2, is a mitochondrial peptide. It plays a role as a neuroprotective factor. It protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Humanin reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.

Protocols

Product Specific Protocols
IHC protocol for humanin antibody 25886-1-APDownload protocol
FC protocol for humanin antibody 25886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Impaired Expression of Humanin during Adrenocortical Carcinoma

Authors - Małgorzata Blatkiewicz
View Article