6*his,His tag Monoklonaler Antikörper

6*his,His tag Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 60001-1-Ig

Synonyme

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Galerie der Validierungsdaten

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant Protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60001-1-Ig bindet in WB, ELISA 6*his,His tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: 6*his,His tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tags are most often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Publikationen

Species	Application	Title
		Nat Commun; Caspr1 is a host receptor for meningitis-causing Escherichia coli.; Authors - Wei-Dong Zhao
	WB	J Exp Bot; Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in Arabidopsis.; Authors - Yao Xuan X
	WB	Oncol Rep; Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma.; Authors - Ying Ji
		J Biol Chem; A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells.; Authors - Shu-Hong Zhang