CD62L (L-Selectin) Monoklonaler Antikörper
CD62L (L-Selectin) Monoklonal Antikörper für FC
Wirt / Isotyp
Ratte / IgG2a
Getestete Reaktivität
Maus
Anwendung
FC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
MEL-14
Kat-Nr. : CL488-65123
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL488-65123 bindet in FC CD62L (L-Selectin) und zeigt Reaktivität mit Maus
| Getestete Reaktivität | Maus |
| Wirt / Isotyp | Ratte / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | C3H/eb-Maus-B-Lymphom 38C-13 |
| Vollständiger Name | selectin, lymphocyte |
| GenBank-Zugangsnummer | BC052681 |
| Gene symbol | Sell |
| Gene ID (NCBI) | 20343 |
| Konjugation | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
| Form | Liquid |
| Reinigungsmethode | Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3. |
| Lagerungsbedingungen | Bei 2-8°C lagern. Vor Licht schützen. |
Hintergrundinformationen
L-selectin, also known as SELL or CD62L, is a member of the selectin family of adhesion molecules that includes E-selectin and P-selectin (PMID: 2663882, 2473156, 1382078). L-selectin is a protein with the calculated molecular mass of 42 kDa but actually 95-105 kDa on neutrophils and 74 kDa on lymphocytes because of the highly glycosylation (PMID: 1694883, 1695155). L-selectin is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). L-selectin mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and are also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
