HNRNPF Monoklonaler Antikörper

HNRNPF Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E7A4

Kat-Nr. : CL488-67701

Synonyme

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

---

Galerie der Validierungsdaten

• 

  FC experiment of HepG2 using CL488-67701

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HNRNPF (CL488-67701, Clone:2E7A4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67701 bindet in FC (Intra) HNRNPF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HNRNPF fusion protein Ag7128
• Vollständiger Name: heterogeneous nuclear ribonucleoprotein F
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001432
• Gene symbol: HNRNPF
• Gene ID (NCBI): 3185
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011