TRAPPC9/NIBP Polyklonaler Antikörper

TRAPPC9/NIBP Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19549-1-AP

Synonyme

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRS120, Tularik gene 1 protein

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 19549-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19549-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Hirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19549-1-AP bindet in WB, IHC,ELISA TRAPPC9/NIBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: trafficking protein particle complex 9
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: NM_031466
• Gene symbol: TRAPPC9
• Gene ID (NCBI): 83696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPPC9, also named NIK- and IKBKB-binding protein (NIBP), is involved in the NF-kappaB signaling pathway and directly interacts with IKK-beta and MAP3K14. TRAPPC9 functions as an activator of NF-kappa-B via increased phosphorylation of the IKK complex. TARPPC9 may also function in neuronal cells differentiation and defects in TRAPPC9 are the cause of mental retardation autosomal recessive type 13 (MRT13), which is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior. TARPPC9 is a component of the multisubunit TRAPP (transport protein particle) complex and it may play a role in vesicular transport from endoplasmic reticulum to Golgi.