Anticorps Monoclonal anti-6*his,His tag

6*his,His tag Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Protéine recombinante

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 60001-1-Ig

Synonymes

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Galerie de données de validation

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Applications testées

• Résultats positifs en WB: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60001-1-Ig cible 6*his,His tag dans les applications de WB, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: 6*his,His tag
• Masse moléculaire calculée: 1 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tags are most often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Publications

Species	Application	Title
		Nat Commun; Caspr1 is a host receptor for meningitis-causing Escherichia coli.; Authors - Wei-Dong Zhao
	WB	J Exp Bot; Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in Arabidopsis.; Authors - Yao Xuan X
	WB	Oncol Rep; Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma.; Authors - Ying Ji
		J Biol Chem; A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells.; Authors - Shu-Hong Zhang