Anticorps Monoclonal anti-CD62L (L-Selectin)
CD62L (L-Selectin) Monoclonal Antibody for FC
Hôte / Isotype
Rat / IgG2a
Réactivité testée
souris
Applications
FC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
MEL-14
N° de cat : CL488-65123
Synonymes
Galerie de données de validation
Applications testées
Dilution recommandée
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-65123 cible CD62L (L-Selectin) dans les applications de FC et montre une réactivité avec des échantillons souris
| Réactivité | souris |
| Hôte / Isotype | Rat / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Lymphome B de souris C3H/eb 38C-13 |
| Nom complet | selectin, lymphocyte |
| Numéro d’acquisition GenBank | BC052681 |
| Symbole du gène | Sell |
| Identification du gène (NCBI) | 20343 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
| Forme | Liquide |
| Méthode de purification | Purification par affinité |
| Tampon de stockage | PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3. |
| Conditions de stockage | Stocker à une température comprise entre 2 °C et 8 °C. Éviter toute exposition à la lumière. |
Informations générales
L-selectin, also known as SELL or CD62L, is a member of the selectin family of adhesion molecules that includes E-selectin and P-selectin (PMID: 2663882, 2473156, 1382078). L-selectin is a protein with the calculated molecular mass of 42 kDa but actually 95-105 kDa on neutrophils and 74 kDa on lymphocytes because of the highly glycosylation (PMID: 1694883, 1695155). L-selectin is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). L-selectin mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and are also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
