Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

rat

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

24F

N° de cat : FITC-65224

Synonymes

B7 2, Cd86, CD86 molecule

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Galerie de données de validation

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  FC experiment of rat splenocytes using FITC-65224

  1X10^6 unstimulated or LPS-treated (5 ug/mL, 3 days) rat splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) and 0.5 ug FITC Plus Anti-Rat CD86 (FITC-65224, Clone: 24F). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS treated rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65224 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HTLV-1-transformed Lewis-S1 rat T cell line
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 36 kDa
• Numéro d’acquisition GenBank: NM_020081
• Symbole du gène: Cd86
• Identification du gène (NCBI): 56822
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.