Anticorps Polyclonal de lapin anti-CENPA

CENPA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 26754-1-AP

Synonymes

Histone H3-like centromeric protein A, CENP-A, CENP A

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Galerie de données de validation

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  WB analysis of MCF-7 using 26754-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26754-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 26754-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26754-1-AP (CENPA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

26754-1-AP cible CENPA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CENPA Protéine recombinante Ag25025
• Nom complet: centromere protein A
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC002703
• Symbole du gène: CENPA
• Identification du gène (NCBI): 1058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Publications

Species	Application	Title
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi
human	WB	Pathol Res Pract; CENPA facilitates glioma stem cell stemness and suppress ferroptosis to accelerate glioblastoma multiforme progression by promoting GBP2 transcription; Authors - Chuankun Li
	IF	Proc Natl Acad Sci U S A; Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta; Authors - Talia Hatkevich

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Adult mouse retina paraffin embedded and Cryo embedded embryonic (E14) mouse. The Cryo embedded tissue stains better with antigen retrieval (citric buffer)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Adult mouse retina, embryonic (E14) mouse retina.

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