Anticorps Monoclonal anti-AGO2
AGO2 Monoclonal Antibody for WB,ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain
Applications
WB,ELISA
Conjugaison
Non conjugué
CloneNo.
2D11C11
N° de cat : 60026-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Human placenta |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
60026-1-Ig cible AGO2 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | AGO2 Protéine recombinante Ag1032 |
| Nom complet | eukaryotic translation initiation factor 2C, 2 |
| Masse moléculaire calculée | 97 kDa |
| Numéro d’acquisition GenBank | BC007633 |
| Symbole du gène | EIF2C2 |
| Identification du gène (NCBI) | 27161 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Précipitation de l'acide caprylique/du sulfate d'ammonium |
| Tampon de stockage | PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
