Anticorps Monoclonal anti-PPARA

• Phare
• Validé par KD/KO

PPARA Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (6)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(123)

CloneNo.

1G1E10

Avis (1)

N° de cat : 66826-1-Ig

Synonymes

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

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Galerie de données de validation

• 

  WB analysis of ROS1728 using 66826-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66826-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules ROS1728

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 116 publications below
• IHC: See 14 publications below
• IF: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

66826-1-Ig cible PPARA dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPARA Protéine recombinante Ag7896
• Nom complet: peroxisome proliferator-activated receptor alpha
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC000052
• Symbole du gène: PPARA
• Identification du gène (NCBI): 5465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
human,mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu ; KD Validated
human	WB,IHC	Cell Death Differ; RUNX2 recruits the NuRD(MTA1)/CRL4B complex to promote breast cancer progression and bone metastasis.; Authors - Xin Yin
rat	WB	Phytomedicine; Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept; Authors - Tao Jiang
human	WB	J Med Chem; Precise Carrier-Free Pt(IV)-Nanobombs for Apoptosis/Ferroptosis Synergistic Tumor Therapy: A New Effective Method to Obtain Good Chemotherapy and Low Toxicity; Authors - Xu Guo
human,mouse	WB	J Agric Food Chem; Exposure to Succinate Leads to Steatosis in Non-Obese Non-Alcoholic Fatty Liver Disease by Inhibiting AMPK/PPARα/FGF21-Dependent Fatty Acid Oxidation; Authors - Hong Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tianyi (Verified Customer) (08-30-2024)

Antigen retrieval in Tris/EDTA buffer and secondary antibody in AF647.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovarian tissue

PPARA Antibody Immunofluorescence validation (1:100 dilution) in Ovarian tissue (Cat no:66826-1-Ig)

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