Anticorps Polyclonal de lapin anti-Phospho-MARCKS (Ser159/163)

Phospho-MARCKS (Ser159/163) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 29103-1-AP

Synonymes

80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, Phospho-MARCKS (Ser159/163), PKCSL, PRKCSL

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Galerie de données de validation

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  WB analysis using 29103-1-AP

  Non-treated NIH/3T3 cells, Serum-free treated and Insulin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29103-1-AP (Phospho-MARCKS (Ser159/163) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: Serum-free treated NIH/3T3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29103-1-AP cible Phospho-MARCKS (Ser159/163) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myristoylated alanine-rich protein kinase C substrate
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC089040
• Symbole du gène: MARCKS
• Identification du gène (NCBI): 4082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Myristoylated Alanine Rich C-Kinase Substrate (MARCKS) is a ubiquitous, highly conserved protein among vertebrates, which is essential for postnatal survival, and has been widely studied for its functions in the brain and nervous system. Being highly expressed in nervous tissue, particularly during early development but persisting in the adult, it plays numerous roles related to brain growth, neuronal migration, neurite outgrowth, neurotransmitter release, and synaptic plasticity. Protein kinase C (PKC) phosphorylates MARCKS, which converts MARCKS from a membrane-bound protein to a cytoplasmic protein. The phosphorylation site of MARCKS protein is called the effector domain (ED). Its structure is highly conserved. It can be combined with cell membrane, PKC, calcium/calmodulin-dependent kineses (CaMK) and F-actin. Studies have shown that increased membrane-bound, non-phosphorylated MARCKS might be conducive to the stabilization of synaptic morphology. Phosphorylated MARCKS protein (P-MARCKS) can regulate the stability of actin network and alter the synaptic structure. (PMID: 30655546, PMID: 30155805)

Publications

Species	Application	Title
human	WB	World J Gastrointest Oncol; LINC01268 promotes epithelial-mesenchymal transition, invasion and metastasis of gastric cancer via the PI3K/Akt signaling pathway and targeting MARCKS; Authors - Ling-Han Tang