KPNA6 Polyclonal antibody

KPNA6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12366-2-AP

Synonyms

Importin subunit alpha 7, IPOA7, Karyopherin subunit alpha 6, KPNA6



Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12366-2-AP targets KPNA6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KPNA6 fusion protein Ag3040
Full Name karyopherin alpha 6 (importin alpha 7)
Calculated Molecular Weight 536 aa, 60 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC020520
Gene Symbol KPNA6
Gene ID (NCBI) 23633
RRIDAB_2281067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KPNA6 antibody 12366-2-APDownload protocol
IHC protocol for KPNA6 antibody 12366-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.

Authors - Sergi Marco
humanWB

Virol Sin

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Authors - Yisha Zhuo
humanWB

Viruses

Ivermectin Inhibits HBV Entry into the Nucleus by Suppressing KPNA2

Authors - Anna Nakanishi