IHC Detect Kit for Rabbit/Mouse Primary Antibody

Sample type

FFPE tissues

Assay type

Immunohistochemistry

Secondary antibody host species

Goat

Secondary antibody species specificity

Rabbit/Mouse

Publications

22

Cat no : PK10006

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Validation Data Gallery

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  IHC analysis of human placenta tissue using Proteintech’s SLC2A3 rabbit polyclonal antibody (20403-1-AP) and IHC Detect Kit for Rabbit/Mouse Primary Antibody (PK10006).

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  IHC analysis of human pancreas cancer tissue using Proteintech’s Insulin mouse monoclonal antibody (66198-1-Ig) and IHC Detect Kit for Rabbit/Mouse Primary Antibody (PK10006).

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Product Information

The PK10006 kit is designed to facilitate signal detection during IHC analysis of FFPE tissue samples using primary antibodies raised in rabbits/mice. The kit consists of Polymer HRP-Goat anti- Rabbit/Mouse Secondary Antibody and ultra-sensitive DAB Chromogen for highly sensitive detection of rabbit/mouse primary antibodies. The kit also contains an optimized primary antibody dilution buffer that preserves primary antibody activity and helps lower non-specific background staining.

Note: 

* If you are planning to use a rabbit primary antibody for staining mouse samples, we recommend using our IHC Detect Kit for Rabbit Primary Antibody (Cat.NO. PK10009). 

* If antigen retrieval buffer, counter staining reagent and mounting media are needed, please see our IHC Prep & Detect Kit for Rabbit/Mouse Primary Antibody (Cat.NO. PK10019).

Components

Part NO.	Component	Size
HC000	Quenching Buffer (RTU)	6 mL
HC004	Primary Antibody Diluent (RTU)	24 mL
HC005	Polymer HRP-Goat anti-Rabbit/Mouse Secondary Antibody	6 mL
HC006	Chromogen Component A	0.8 mL
HC007	Chromogen Component B	8 mL

Size: 60 tests (may vary depending on tissue size).

Storage

All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Usage

IHC Detect Kit for Rabbit/Mouse Primary Antibody Data Sheet

Application Note

This kit is recommended for FFPE tissues and not for frozen sections.

Safety Data Sheet

IHC Detect Kit for Rabbit/Mouse Primary Antibody Safety Data Sheet(SDS)

Publications

Application	Title
	Front Immunol; Glycyrrhetinic acid regulates impaired macrophage autophagic flux in the treatment of non-alcoholic fatty liver disease; Authors - Yadong Fan
	Adv Sci (Weinh); Self-Activated Cascade-Responsive Sorafenib and USP22 shRNA Co-Delivery System for Synergetic Hepatocellular Carcinoma Therapy.; Authors - Shengjun Xu
IHC	Zhongguo Fei Ai Za Zhi; [Expression and Diagnostic Value of NPTX1 in Thymoma Patients].; Authors - Xin Du
	Cell Death Dis; Prevention of D-GalN/LPS-induced ALI by 18β-glycyrrhetinic acid through PXR-mediated inhibition of autophagy degradation.; Authors - Shouyan Wu
IHC	Adv Sci (Weinh); RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation.; Authors - Ziyang Wang
	Adv Sci (Weinh); Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer.; Authors - Quan Liu