CoraLite®647 Anti-Human CD107b (H4B4)

CD107b Monoclonal Antibody for IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF,FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

H4B4

Cat no : CL647-65053

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 65053-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65053 targets CD107b in IF,FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for CL647 CD107b antibody CL647-65053Download protocol
FC protocol for CL647 CD107b antibody CL647-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols