Anti-Human CD226 Rabbit Recombinant Antibody

CD226 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230041A3

Cat no : 98002-1-RR

Synonyms

CD226, CD226 antigen, CD226 molecule, DNAM 1, DNAM1, DNAX accessory molecule 1, PTA1, TLiSA1

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 98002-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-RR, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98002-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-RR, Clone:41A3) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98002-1-RR

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD226 Rabbit Recombinant Antibody (98002-1-RR, Clone:41A3) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000; or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:11A8) (center) or 0.2 ug Anti-Human CD226 Mouse Monoclonal Antibody (Clone:DX11) (right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity experiments of 98002-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98002-1-RR against Human CD226 were performed. The affinity constant is 37.1 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98002-1-RR targets CD226 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD226 molecule
• Calculated Molecular Weight: 336 aa, 39 kDa
• GenBank Accession Number: BC074787
• Gene Symbol: CD226
• Gene ID (NCBI): 10666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).