Various lysates were subjected to SDS PAGE followed by western blot with 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB result of PPAR Gamma antibody (60127-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAR Gamma transfected HL-60 cells.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1x10^6 HeLa cells were intracellularly stained with 0.4 ug PPAR Gamma Monoclonal antibody (60127-1-Ig, Clone:4E12F10)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).