PPAR Gamma Monoclonal antibody

• Featured Product
• KD/KO Validated

PPAR Gamma Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

4E12F10

Cat no : 60127-1-Ig

Synonyms

CIMT1, NR1C3, PPAR gamma, PPARG1, PPARγ

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Validation Data Gallery

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  WB analysis using 60127-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 60127-1-Ig

  WB result of PPAR Gamma antibody (60127-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAR Gamma transfected HL-60 cells.

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  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 60127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60127-1-Ig (PPAR Gamma antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon using 60127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using PPAR Gamma antibody (60127-1-Ig, Clone: 4E12F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60127-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug PPAR Gamma Monoclonal antibody (60127-1-Ig, Clone:4E12F10)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human colon tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

60127-1-Ig targets PPAR Gamma in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, sheep
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPAR Gamma fusion protein Ag10005
• Full Name: peroxisome proliferator-activated receptor gamma
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC006811
• Gene Symbol: PPARG
• Gene ID (NCBI): 5468
• RRID: AB_2166065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publications

Species	Application	Title
mouse	WB	J Physiol Biochem; miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2.; Authors - Chen Chen
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
mouse	IHC	J Immunol Res; Identification of Adipogenesis Subgroups and Immune Infiltration Characteristics in Diabetic Peripheral Neuropathy; Authors - Yumin Lin
sheep	WB	Int J Mol Sci; The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432; Authors - Meilin Jin