Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 Cytokeratin 7 antibody (CL488-15539) at dilution of 1:400, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, red), CoraLite®594 CD31 antibody (CL594-11265, red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 7 (CL488-15539) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).