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COG6 Polyclonal antibody

COG6 Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14277-1-AP

Synonyms

COD2, COG complex subunit 6, COG6, KIAA1134

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis using 14277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis of human brain using 14277-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using COG6 Polyclonal antibody (14277-1-AP)

    WB analysis of NIH/3T3 using 14277-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using COG6 Polyclonal antibody (14277-1-AP)

    IF Staining of MCF-7 using 14277-1-AP

    Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COG6 Polyclonal antibody (14277-1-AP)

    IF Staining of HeLa using 14277-1-AP

    Immunofluorescent analysis of Hela cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HeLa cells using COG6 Polyclonal antibody (14277-1-AP)

    FC experiment of HeLa using 14277-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COG6 (14277-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IF detected inMCF-7 cells, Hela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14277-1-AP targets COG6 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG6 fusion protein Ag5624
Full Name component of oligomeric golgi complex 6
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC051723
Gene Symbol COG6
Gene ID (NCBI) 57511
RRIDAB_10694426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COG6 antibody 14277-1-APDownload protocol
IF protocol for COG6 antibody 14277-1-APDownload protocol
FC protocol for COG6 antibody 14277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols