• Featured Product
  • KD/KO Validated

XPG Polyclonal antibody

XPG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11331-1-AP

Synonyms

ERCC5, ERCM2, EC:3.1.-.-, DNA repair protein complementing XP-G cells, DNA excision repair protein ERCC-5



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Daudi cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11331-1-AP targets XPG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XPG fusion protein Ag1874
Full Name excision repair cross-complementing rodent repair deficiency, complementation group 5
Calculated Molecular Weight 1186 aa, 133 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC031522
Gene Symbol ERCC5
Gene ID (NCBI) 2073
RRIDAB_2098155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.

Protocols

Product Specific Protocols
WB protocol for XPG antibody 11331-1-APDownload protocol
IF protocol for XPG antibody 11331-1-APDownload protocol
IP protocol for XPG antibody 11331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.

Authors - Takaaki Yasuhara
  • KD Validated
human

Cancer Cell

Systematic Functional Annotation of Somatic Mutations in Cancer.

Authors - Patrick Kwok-Shing Ng

Mol Carcinog

Associations between Expression Levels of Nucleotide Excision Repair Proteins in Lymphoblastoid Cells and Risk of Squamous Cell Carcinoma of the Head and Neck.

Authors - Peng Han
humanWB

Oncol Rep

Characterization of the nucleotide excision repair pathway and evaluation of compounds for overcoming the cisplatin resistance of non‑small cell lung cancer cell lines.

Authors - Toshihiro Suzuki
  • KD Validated
humanWB

J Cancer

PMID: 31772670

Authors - Li-Ming Tan
humanWB

Biochim Biophys Acta

Stimulation of ribosomal RNA gene promoter by transcription factor Sp1 involves active DNA demethylation by Gadd45-NER pathway.

Authors - Pallavi Rajput