Uni-rAb

Recombinant Monoclonal Antibodies  

Uni-rAb antibodies are the latest generation of recombinant monoclonal antibodies developed using Proteintech’s Antigen-Specific B-Cell Cloning & Engineering (ABCE) platform. The ABCE platform achieves stable and high expression of recombinant monoclonal antibodies by cloning antibody heavy and light chain sequences derived from antigen-specific B cells into high-yield expression vectors followed by their introduction into suitable mammalian expression systems.

Uni-rAb antibodies are the latest generation of recombinant monoclonal antibodies developed using Proteintech’s Antigen-Specific B-Cell Cloning & Engineering (ABCE) platform. The ABCE platform achieves stable and high expression of recombinant monoclonal antibodies by cloning antibody heavy and light chain sequences derived from antigen-specific B cells into high-yield expression vectors followed by their introduction into suitable mammalian expression systems.

  • Monoclonal antibodies with native VH-VL pairings
  • Unparalleled lot-to-lot consistency​
  • Excellent specificity & high affinity
  • High-throughput production
  • High purity
  • Available in carrier-free formulations

ABCE Platform

Leveraging more than 20 years of antibody development experience, Proteintech’s ABCE platform combines the in-depth technical expertise of our R&D team with recent advancements in antibody technology and cutting-edge automation to ensure rapid and high-throughout production of a variety of off-the-shelf as well as custom recombinant antibodies.

Rabbit recombinant monoclonal antibody development process

Uni-rAb recombinant antibody development workflow on the ABCE platform.

Advantages

Precise antigen design  

Generating precise and high-quality antigens plays a critical role in the successful development of antibodies. As a world-renowned manufacturer of antibodies, Proteintech has developed a vast portfolio of over 15,000 rabbit polyclonal and mouse monoclonal antibodies covering almost 2/3 of the human proteome. Antigens that were used for the development of these antibodies have been already verified and therefore, can serve as valuable raw materials for the rapid generation of Uni-rAb recombinant antibodies. Moreover, our scientists bring decades of experience to help you generate antigens tailored to the specific needs of your project, including antigens for post-translational modifications such as phosphorylation, acetylation, etc. or challenging targets such as membrane proteins.

Antigen-specific single B cell sorting ​

Antigen-specific single B cell sorting allows the isolation of functional monoclonal antibodies reactive against antigens in their native conformation that predominantly occur in vivo and are difficult to reproduce in vitro. This ultimately leads to the generation of Uni-rAb recombinant antibodies that have native VH-VL pairings which is critical for maintaining antibody specificity and functionality but is often lost in other antibody development approaches such as phage display. The ABCE platform utilizes antigen dual-labeling and multiparametric FACS to exclude low-affinity and non-specific B cells, thereby facilitating the isolation of antigen-specific B cells with high efficiency.

Phospho-Histone H2A.X (Ser139) Recombinant antibody​

Antigen-specific single B cells sorting results in a high percentage of positive clones as verified by ELISA.

Antigen-specific single B cells sorting results in a high percentage of positive clones as verified by ELISA.

Rigorously screened and validated ​

Uni-rAb recombinant antibodies are rigorously screened and validated to ensure the highest level of performance across multiple applications. All Uni-rAb antibodies undergo screening at three different stages during their development, including: 1) high-throughput initial supernatant screening, 2) high-throughput re-validation of purified antibody, and 3) final functional validation prior to the formal release of the antibody. Screenings are conducted using various immunoassays, including Western blot (WB), immunofluorescence (IF), and flow cytometry (FC) and the screening data obtained at the three stages are carefully analyzed to confirm correlation.

Moreover, Uni-rAb recombinant antibodies are subjected to a label-free biomolecular interaction analyzer which uses Biomembrane interferometry (BLI) technology to detect the affinity index between antibodies and antigens. Majority of Uni-rAb recombinant antibodies have been validated to have dissociation constants <10-10.

Uni-rAB recombinant monoclonal antibodies are screened at three different stages during their development on the ABCE platform.

Uni-rAb antibodies are screened at three different stages during their development on the ABCE platform.

98005-1-RR (Mouse IL-17A) ​

Measurement of Uni-rAb antibody affinity for 98005-1-RR

83188-2-RR (Human MMP13) ​

Measurement of Uni-rAb antibody affinity for 83188-2-RR

Measurement of Uni-rAb antibody affinity using biolayer interferometry (BLI). ​

Two-step purification​

Uni-rAb recombinant antibodies are purified via a two-step purification process, which includes protein A purification followed by mixed-mode cation/anion exchange chromatography. This helps reduce the impact of residual impurities such as nucleases, host proteins, shed Protein A, antibody aggregates and invalid antibody fragments on antibody quality, ensuring high purity and consistency between different batches of Uni-rAb antibodies.

Two-step purification phospho PDH E1 Alpha (Ser 232) recombinant antibody

Two-step purification of Uni-rAb recombinant antibodies. ​

Unparalleled lot-to-lot consistency​

Since the development of Uni-rAb recombinant monoclonal antibodies relies on the in vitro cloning and expression of defined heavy and light chain sequences derived from antigen-specific B cells, the entire production process can be standardized and replicated whenever needed once the antibody sequences are known. Being biologically defined eliminates the risks of genetic drift and instability as seen in traditional hybridoma-derived monoclonal antibodies, thereby ensuring unparalleled lot-to-lot consistency and reproducibility of experimental data.

Rabbit recombinant monoclonal antibody development process

Western blot analysis of various cell lysates using three separate batches of Uni-rAb Beta Actin Recombinant Antibody (81115-1-RR).

​High-throughput production 

Single B cell-based antibody development offers a highly efficient platform for the rapid generation of recombinant antibodies with excellent specificity against any antigen of choice. Higher number of positive clones with natural VH-VL pairings can be obtained from a small number of cells and in a much shorter time compared to traditional hybridoma-based or phage display approaches. For targets with pre-qualified antigens from our rabbit polyclonal and mouse monoclonal antibody development pipelines, lead candidates for recombinant Uni-rAb antibody production can be identified in as fast as 4 weeks.

Instruments for high-throughput production

State-of-the art instrumentation utilized on the ABCE platform. ​

Validation Data

Uni-rAb Categories

Unconjugated Uni-rAb Antibodies

Wide collection of unconjugated monoclonal recombinant antibodies against key targets covering multiple areas of research and validated in multiple applications.

Conjugated Uni-rAb Antibodies

Monoclonal recombinant antibodies conjugated to Proteintech’s proprietary range of CoraLite Plus dyes, perfect for IF and FC applications.

PBS-Only Uni-rAb Antibodies

Monoclonal recombinant antibodies free of carriers including BSA, sodium azide and glycerol offering the flexibility for use in various downstream applications including conjugation and mass cytometry.

Uni-rAb Antibodies for Flow Cytometry

Monoclonal recombinant antibodies against popular CD markers, cytokines and intracellular targets optimized for flow cytometry.

Uni-rAb Matched Antibody Pairs

Matched recombinant antibody pairs consisting of a capture antibody and a detection antibody specific for different epitopes on the same antigen.

All Uni-rAb Antibodies​

Explore our rapidly expanding portfolio of all monoclonal recombinant antibodies with hundreds of new targets being added every month.

Custom Services

If you are interested in custom antibody development services, look no further. Proteintech’s scientists bring over 20 years of experience in antigen design, expression and purification to help you with your custom antibody development project. Our ABCE platform offers the flexibility of multi-species antibody discovery for desired targets with a rapid turnaround

Projected timeline for custom antibody development on the ABCE platform workflow graphic ​

Projected timeline for custom antibody development on the ABCE platform. ​

In addition to full-length antibodies, we can develop a variety of custom antibodies tailored to specific project needs, including scFv antibodies, Fc fusion antibodies, Fc mute and other Fc modifications, chimeric antibodies, bispecific antibodies and antibody fragments such as Fab and F(ab’)2. ​

customized recombinant antibody

Types of custom antibodies that can be developed by Proteintech.​

Proteintech can help you with a wide range of custom antibody services as listed below.

Single B Cell Services Available for

  • Rabbit
  • Mouse
  • Rat
  • Human
  • Alpaca
  • Sheep
  • Chicken
  • Swine
  • Dog
  • Cat
  • Horse

Antibody Engineering Services

  • Fab, F(ab’) fragments​
  • scFv, scFv-Fc, VHH-Fc
  • Fc mute & other Fc modifications
  • Chimeric antibodies​
  • Bi-specific antibodies​
  • PTM-specific antibodies​
  • Antibody humanization
  • Antibody conjugation including site-specific and stoichiometric conjugation
  • Antibody-drug conjugates

Antibody Characterization ​Services

  • ELISA and antibody pairing​
  • FACS binding and blocking​
  • Functional assays including WB, IP, IHC, IF & FC​
  • Neutralization test​
  • Affinity measurement​
  • EC50/IC50 measurement

Talk to Our Technical Experts ​

women scientist pipetting